Slight miss when cruising

Not sure if this is a misfire but when cruising on freeway roughly 60-70 mph 2700 rpm I feel a slight hesitation not major just enough to annoy me where should I look first?? ... Now mind you this is a 410 stroker 4 speed 3:23 gear .. Holley 850 dp and dizzy is locked out no mechanical advance locked at 35 degrees ... Now when I floor it ... It goes hard ..no knocking or anything when I punch it just cruising I can feel a mis and hear it in the exhaust ...running 91 octane

Would the 5/16 fuel line be part of that issue?? Have yet switched to my 3/8 line

Thanks guys