You can either leave that bolt out, or use the bolt with the hole...
As long as the hole is open and able to let oil get to the timing chain...
If you have the drip tab for oiling, it requires leaving the bottom driver's side bolt out for the locating tab... I would not recommend not having the other bolt left out, but use the bolt with the hole in it as I wouldn't trust only 2 bolts to hold the camshaft thrust plate...